4D Quantitative Microscopy
Real-Time 3D Imaging With Differential Digital Holography Microscopy
The ‘Microgravity Research Center’ (MRC) of the “Université Libre de Bruxelles” (ULB) and Ovizio Imaging Systems have developed a unique holographic microscopy technology based on several years of research and a broad portfolio of patents.
In traditional light microscopy only intensity information is recorded. ‘Digital Holographic Microscopy’ (DHM) also stores data that is relevant to the optical phase or indirectly to the optical thickness of a sample. This allows the development of quantitative imaging applications and full automation of analyses.
‘Digital Holographic Microscopy’ distinguishes itself from other microscopy methods by not recording the projected image of the object. Instead, the light wave front information originating from the object is digitally recorded as a hologram, from which a computer calculates the object image using a numerical reconstruction algorithm. In digital holography, a light source, in most cases a laser emitting a coherent beam,is used to illuminate an object or sample. This beam is split in an interferometer into a reference beam and an object beam. The two light paths interfere on a CCD camera creating a hologram. The light wave front information resulting from the interference process is used to digitally reconstruct the object image using an advanced software algorithm, thus replacing the lens in a traditional light microscope.
Ovizio’s ‘Differential Digital Holographic Microscopy’, patented by the ULB, is an evolution of this base technology bringing increased stability, flexibility and an important reduction of size of the instruments while using lower cost, non-invasive LED light sources.