Capturing the Most From Your Suspension Cell Culture Process. In Real-Time.
The iLine F microscope uses a unique concept for in-line, non-invasive and label-free suspension cell counting, offering its users an unmatched experience when combined with a bioreactor. Continuously tracking cells in real-time with high accuracy, the system has been designed for a straightforward experience and a particularly efficient tool for deep investigation of underlying data. Fast and accurate, the algorithms automatically discriminate living from dead cells, count clusters and eliminate debris. As a result : No manual operations and No toxic waste.
The microscope captures holograms of the cells travelling through the BioConnect disposable fluidics system, which connects the microscope to the bioreactor. The fluidics system is activated via a unique membrane pump, applying the principle of a heart and proven not to affect cells, while the closed loop set-up ensures sterility and continuous viable cell counting and monitoring without sample consumption.
Transfer of OVIZIO’s technology from one site to another becomes very convenient (not operator dependent) and much less time consuming.
Cost comparison with traditional counters showed that the iLine system enables to decrease yearly operational costs by 40% in case of medium-sized facilities and up to 50% for large facilities. The system is straightforwardly scalable, and can be used from R&D to process development and production.
The system can track the following parameters:
- Cell Density
- Cell Viability
- Cell Morphology
- Feeding and Harvesting Time
- Discriminate Living From Dead Cells
iLine F microscope can be adapted to most stirred tank bioreactors through an available standard PG-13.5 port.