Lipid Accumulation in Adipocytes & Macrophages
a) 3T3-L1 pre-adipocytes and 3T3-L1 adipocytes:
Label-free 3D Holographic Imaging for studying lipid accumulation in adipocytes. The mature adipocytes contain significantly more lipids compared to the pre-adipocytes. Compared to classic microscopy which requires sampling, fixing & staining, OVIZIO's approach enables the observation of samples without alteration, making them available for further studies (e.g. Time Lapse Studies).
3-D Phase Images (40x)
Murine pre-adipocytes / Murine adipocytes
b) Murine Raw 264.7 Macrophages cultivated in 24-well plates:
Macrophage cellular morphology observed via 3D imaging to enable label-free analysis of lipid accumulation. Murine macrophages were incubated for 22 hours with native low-density lipoprotein (LDL) (left). Picture on the right shows macrophages incubated with myeloperoxidase-oxidized LDL (mox LDL).
3 D Phase Image (40x)
Incubated with Native LDL / Incubated with Mox LDL
Source: results obtained in the context of the Walonia Region BioWin Bioline project, UNamur, Martine Raes.