Label free recognition of non activated and activated human T cells by Quantitive Phase Imaging
There is a gap in current methods for evaluating quality and durability of cells in immunotherapeutic products like CAR-T.
Ability to identify and characterize immune cells on single-cell level without labelling would be highly valuable for biomedical research and clinical trials where unmodified cells are required.
We are developing analytical applications for label-free monitoring of human T cells activation and viral transduction by optical means using image based cell profiling methods.
An application for detection of T cells activation by Quantative Phase Imaging (QPI) was developed. We showed that stimulated cells could be statistically distinguished from non-stimulated by optical morphology fingerprints obtained from their phase images.
Miniaturization of cell bioprocessing platforms and performing cell Quality Control (QC) analysis in a fast and low-cost way would be a benefit. Microfluidic devices were tested and Quantative Phase Imaging was applied for detecting of non-activated and activated T cells in microfluidic chip. This presents a first step towards high throughput analytical platforms for CAR-T cells.