iLine F

Capturing the Most From Your Suspension Cell Culture Process. In Real-Time.

QMod

Holographic And Fluorescence Microscopy. In One Single System.

iLine M - Engineering Platform

Customised Cell Confluence Monitoring In Multi-Layer Vessels.

Label free recognition of non activated and activated human T cells by Quantitive Phase Imaging

There is a gap in current methods for evaluating quality and durability of cells  in immunotherapeutic products like CAR-T. 

Ability to identify and characterize immune cells on single-cell level without labelling would be highly valuable for biomedical research and clinical trials where unmodified cells are required.

We are developing analytical applications for label-free monitoring of human T cells activation and viral transduction by optical means using image based cell profiling methods. 

An application for detection of T cells activation by Quantative Phase Imaging (QPI) was developed. We showed that stimulated cells could be statistically distinguished from non-stimulated by optical morphology fingerprints obtained from their phase images. 

Miniaturization of cell bioprocessing platforms and performing cell Quality Control (QC) analysis in a fast and low-cost way would be a benefit. Microfluidic devices were tested and Quantative Phase Imaging was applied for detecting of non-activated and activated T cells in microfluidic chip. This presents a first step towards high throughput analytical platforms for CAR-T cells.

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