Cell Counting on Microbeads
CHO cells - on treated polystyrene microcarriers in disposable spinner flask.
With the Digital Holography it becomes possible to detect cells on the surface of translucent microcarriers such as Cytodex 3 (Sigma-Aldrich) and count these cells based on their 'holographic fingerprint'. Images captured with a DHM microscope show that, compared to brightfield microscopy, holography offers the possibility to refocus the holograms post acquisition (see image: 'Refocused') and by doing so, identify cells from debris. When healthy cells are illuminated, they act as lenses and these light points are applied for label-free cell counting on micro-carriers (see images 'zoom on intensity'). Images are processed by Pr. Frank Dubois (Micro Gravity Research Centre, Free University of Brussels) based on the patent pending cell counting algorithm (WO2012062805).
Brightfield image / Phase image / Refocused cells
Intensity peaks / Zoom on intensity peaks / Separated cells
Results of the Waleo project DECISIV, obtained by Professors Frank Dubois and John Wérenne, Daniel Ribeiro, Andreia Pedregal and Dr. Catherine Yourassowsky (Université libre de Bruxelles).